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Question: Gel filtration column (60 mL) was calibrated with blue dextran, its elution volume found to be 17.4 mL. Then, unknown protein and BSA (MW 66 kDa) were ran through the same column and eluted in 43 mL and 36 mL correspondingly. a) what is the void volume for the column? […]

Suppose that you are given a mixture of proteins that you analyze by standard 2-D electrophoresis, with the following results for isoelectric points and apparent molecular weights: protein A (Mr 34,325; pI 7.82), protein B (Mr 65,605; pI 5.44), and protein C (Mr 104,271; pI 6.54).a. In the isoelectric focusing, […]

Quiz 1. Backward effects of left-sided heart failure include: A. Pulmonary congestionB. Jugular vein distentionC. Dependent edema in the legsD. Bounding pulses 2. In performing a physical assessment, the nurse notes the patient has a “barrel” configuration to the chest. This is a consequence of:A. Reduced intrapleural pressuresB. Bronchial airway […]

Oral contraceptives (“birth control pills’) contain a mix of synthetic estrogen and progesterone. a) Based on your knowledge of the ovulatory cycle, explain how this prevents ovulation at the level of hormone activity. b) Usually a monthly dose contains 3 weeks of daily pills with active ingredients and then a […]