1. What part of the Boil buffer helps “break open” the bacterial cells? 2. What is the difference between bacterial genomic DNA and plasmid DNA that results in the precipitation of genomic DNA with proteins, leaving the plasmid in the supernatant? 3. Restriction endonucleases are especially useful if they generate […]

Aniridia is a type of blindness that is due to a dominant gene. Migraines are the result of a different dominant gene. Suppose that a woman who is not blind, but whose parents both suffer from aniridia, goes to a genetic expert for advise. She suffers from migraines, which her […]

Q. A train is travelling up a 3.73 degree incline at a speed of 3.25m/s as the last car breaks free. How far does last car travel before its speed momentarily reaches zero? Q. David is driving a steady 25 while he passes Tina, who is sitting in her car […]

A student runs an enzyme assay using pig heart mitochondrial malate dehydrogenase (MDH; found inthe Krebs cycle). The assay contains the following: 400 ?l 20 mM MOPS, pH 7.4 10 ?l 10 mM NADH (in H2O) 10 ?l 25 mM oxaloacetate (in H2O) 30 ?l of their MDH solution, in […]