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Question: You are studying the promoter of the rpoA gene in E.coli, which has a -35 consensus sequence of TTGACA. If you mutate the -35 sequence to TTCAGA, will this have any effect on the transcription of the rpoA gene? Why or why not?Question: You create a prokaryotic in vitro transcription […]

I am conducting an activity assay to assess the Enzymatic Hydrolysis of 4-Nitrophenyl ?-D-Glucopyranoside by ?-D-Glucosidase. I am happy with the assay, however one of the components is bovine serum albumin ( 0.2% solution in phosphate buffer, to which my enzyme solution is added). I initially assumed that the BSA […]

Question: Microbes are all around us and are controlled and exploited by man for food, drink and fuel production, sewage disposal, degradation of dead animal and plant cells, and a number of other vital life processes. Choose 6 microbes that are either of benefit or cause spoilage within the brewing […]

The brain is a terrible thing to waste. That is why Harvard has set up a centralized Brain Tissue Resource Center it calls The Brain Bank. The Brain Bank specializes in researching how the nervous system functions by analyzing real human brains. Brains donated to the Brain Bank have provided […]