Cells of the adrenal gland and cells in the ovaries produce cortisol and estrogen respectively, staring with the same initial substrate. Both types of cells produce the intermediate compound progesterone. In the adrenal gland, progesterone is converted directly to cortisol. In contrast the cells of the ovaries do not convert […]

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1. Why does Synechocystis not require electroporation or chemical pretreatment prior to transformation?2. What specific genetic manipulation does Synechocystis readily allow that E. coli does not?3. For transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG – 11 plate with glucose, and […]

Mitochondrial ATP synthase comprises of several proteins, and some of these proteins are encoded by the genes in nucleus. One of these proteins is called S. A mutation in S gene deletes 21 base pairs which are immediately 3′ to the start codon. The mutant S gene is transcribed and […]