Botulinus toxin causes paralysis. It binds to presynaptic sites at the motor end plate (neuromuscular junction) and prevents the exocytotic release of transmitter from vesicles. Predict the consequences (increase, decrease, no change & explain why) of blocking the release of acetylcholine at the neuromuscular junction on the following: 1. Presynaptic […]

RNAse is an enzyme that cleaves the P–O^5′ bond in RNA. it has two His residues in the active site. Suggest a plausible explanation why the enzyme activity changes when pH is increased or decreased from ~pH 6.0, Choose all the true statements a.) the RNAse reaction is an example […]

1) Determine where are the hydrophobic or hydrophilic residues concentrated? Where does your mutation fall relative to these clusters and to the inside/outside of the protein? 2)What (if any) are the primary partner(s) or binding site component(s) [such as a heme, DNA, GSH (Glutathione)? It should be obvious to you […]

2. You isolate a Gram positive alkalinophile that grows at a pH = 10. At this external pH, the intracellular pH is maintained at 7.3. Using the equation proton motive force (pmf), determine the contributions of the electrical and chemical energy components to the pmf in this bacterium.  3. You […]