Phenyl-Sepharose resin consiss of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? […]

Assignment Instructions: 1. Discuss the function in detail of each of the following RNA polymerase II transcription factors. TFIIB TFIIE TFIIF TFIIH 2. What additional role does one of the subunits of TFIIH have during the initiation phase that involves other protein kinases? What is the end result? 3. Discuss […]

1. Explain why it is notable that Fts-Zproteins have been found in mitochondria andchloroplasts. 2. What is a hierarchical organization? And I need an example 3. What are the similarities and differences in the cell walls and cell membranes of gram positive and gram negative bacteria? 4. Explain why clinical […]

-Why does E. coli have several different sigma factors? a) they allow different RNA polymerases to bind to the promoters b) they allow the different subunits of the RNA polymerase holoenzyme to bind to each other c) there is no good reason, they all perform the same function d) one […]