1) A wild-type polypeptideconsists of 27 amino acids. Many mutants have been discovered that produce versions of this polypeptide that are longer than normal.These mutations are each due to a substitution of a singlebase. a. What kind of mutation isresponsible? b. Do you expect the first 27amino acids in the […]

A 25 year old man was brought to the emergency room with a broken leg. His medical history was unremarkable, although he reveals that his mother had died of some “complication” following routine surgery. The patient was taken to surgery for repair of his fractured left distal tibia. He was […]

You have two enzymes, 1 and 2. Both convert substance A into substance B. 1 is inhibited by B, because B partially blocks the active site and will not allow more A to enter.  2 is inhibited by a third substance, C, which binds to the back of 2 and changes […]

Phenyl-Sepharose resin consiss of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? […]