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A plasmid 3 Kb in length contains a gene for ampicillin resistance and a gene for tetracycline resistance. The plasmid has a single site for each of EcoRI, Bg/II, and PstI. If DNA is cloned into the EcoRI site, resistance to either antibiotic is not affected. DNA cloned into the […]

In the naturally occurring variant hemoglobin Rainier, Tyr145? is replaced with a Cys. This mutation allows for disulfide bond formation with a nearby Cys in the same subunit. a. By examining the structure of hemoglobin (Pdb file = 1HGA), suggestwhich Cys makes this new disulfide. b. Formation of this disulfide […]

After insolating different species of bacteria, you see two distinct types of colonies. One has a medium-sized shimy colonies that are mucoid with entire margins, convex elevation and a whitish traslucent appearance. This colony type is the dominant type on the plate. The othe has circular, pinhead colonies which are […]

1. a. Draw the structure of dihydroxyacetone (show all bonds). b. Draw the structure again (showing all bonds), and this time indicate the H bonding of dihydroxyacetone to water. Remember which atoms form H bonds — you’ve seen this “rule” several times. Also, each (uncharged) oxygen atom participates in 2 […]