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Suppose you suspect that there is more than one type of subunit in the native molecule. So you treat the native molecule with mercaptoethanol and urea and try to separate the denatured protein into several components. If there really are 2 (or more) different types of subunits, which of the […]

Hans had had a strep throat two weeks before he was to start anew job. He had to complete some work before he could resign fromhis old position so he ignored the bacterial infection. In thefirst week of his new job he noticed he felt weak and short ofbreath. An […]

Phenyl-Sepharose resin consists of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine. Would you apply these polypeptides in a buffer containing high ionic strength or low ionic strength? […]

One-hundred grams of fresh tomatoes were placed in a stomacher and blended for 45 seconds. A 1 mL sample of blended tomatoes was placed in tube containing 9 mL of Trypticase Soy Broth, and incubated at 37C for 24 hours. After the incubation period, 1 L and 10 L loops […]