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1. Why is it neccessary to digest a plasmid with individualrestriciton enzymes first, then follow with a combination ofdigestions? 2, How is the number of fragments produced associated with the numberof cut-sites available to a restriction enzyme? 3. How could one change in a DNA nucleotide alter the formation ofthe […]

Nutrition Assignment Carbs, Fats and Proteins are the 3 “energy yielding” nutrients of the 6 classes of Nutrients. They are referred to as the “Macro-nutrients” as we need more of these than we do vitamins and/or minerals. For this post, please address the following questions. Be sure to post the […]

A few amino acids are post-translationally removed from the C-terminal end of the beta-lactamase enzyme from B. imaginarium (i.e. after translation and release from the ribosome, a protease chews off a few amino acids). The pruified wildtype enzyme is 327 amino acids in length and the sequence of its C-terminal […]

A purified BamHI-cut DNA fragment is end-labeled (i.e. only the terminal 5′ phosphate from each strand is labelled) and partially digested with the enzyme DpnI. The reaction conditions were such that each DNA molecule, if cut at all, is cut only once. The resulting DNA fragments were electrophoresed on an […]