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For many experiments, it is desirable to have a population of cells that are traversing the cell cycle synchronously. One of the first, and still often used, methods for synchronizing cells is the so-called doubled thymidine block. When high concentrations of thymidine are added to the culture fluid, cells in […]

Mitochondria carrying out oxidative phosphorylation consume oxygen. a) What happens to oxygen in mitochondria? b) Describe the effect of an uncoupling agent such as 2,4-dinitrophenol on the rate of oxygen consumption. Assume there is a sufficient supply of oxidizable substrate, ADP, and Pi. c) It was observed that if a […]

1) Anatomical Terminology Stump your classmates! This week, we are learning a new language, the language of anatomy! As with any new language, the best way to learn it is to use it! Practice by describing an injury or illness in anatomical terms and challenging your classmates to “translate” into […]

This question involves Drosophila. Order and give the map distance for these three loci involved in this question: wingless (w) legless (l) buggy (b) The phenotypes indicated above are recessive. A cross between a wild-type female and a male that expresses all of the above recessive phenotypes gives the following […]