Question: You are performing a reverse transcription (RT) reactions for high-throughput analysis using a 96-well plate format. Each 96-well plate is 8 horizontal rows of wells by 12 vertical columns of wells. You need to set up the entire 96-well plate will identical RT components except for the particular primers that […]

Q. An alien from a new planet landed on earth. He is attentive by cars and is determined to figure out how they work. He decides to disassemble one of them and inspect each part independently. He removes one of the tires and proceeds to study all he can about […]

A kan-r, tet-r plasmid is treated with the restriction enzymeBglI, which cleaves the kan (kanamycin) gene. The DNA is annealed and ligated to a BglI digest of Neurospora DNA and thenused to transform E. coli. a. What anitbiotic would you put in the growth medium to ensure that each colony […]

Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: enzymes are proteins.) To complete this project, it may be useful for you to review the Scientific Method Tutorial (found the Course Content section of the classroom under the Science Learning Center […]