Q1. A biologist discovers an alga that is marine, multicellular, and lives at a depth reached only by blue light. This alga probably belongs to which group?

Q2. When cloning a gene into an expression vector, people frequently use two different restriction enzymes so that passenger DNA can be inserted in the correct orientation with respect to promoter sequence present in the plasmid. But orientation is almost certainly less important when producing an interfering RNA. Why is this orientation less important in this case?