Q. After Drosophila DNA has been treated with a restriction enzyme, the fragments are attached to plasmids and selected as clones in E. coli. By using this “shotgun” technique, David Hogness recovered every DNA sequence of Drosophila in a library.
a. How would you identify the clone that contains DNA encoding the protein actin, whose amino acid sequence is known?
b. How would you identify a clone encoding a specific tRNA