IMAGE – 1, 2, 3 is child, 4, 5, is parents

If child has same gel positioning as father, would it be likely for the child to get cancer if the father has it? For the children without the mutation, what is the risk of getting cancer?

Why was a reaction set up using DNA known to contain the sane mutation present in the father’s DNA?

Why was a reaction set up using DNA known to contain the normal (wild-type) sequence? Why was a reaction set up using no template DNA?