1. You have discovered a new species of bacteria. You are able to transfer F factor to the chromosome of this bacteria in two strains (2 different insertions). Both of these strains are a+, b+, c-, d+, e-. An F- strain is a-, b-, c+, d-, e+. You look at the frequency of transfer and gather the following data for the 2 strains.

A) What is the order of the genes in the genome?

B) Explain the difference between the 2 Hfr strains.

Frequency of transfer with the following donors.

Strain 1 Strain 2

a 22% 17%

b 13% 34%

c 6% 47%

d 29% 3%

e 9% 41%