What is the limit of resolution on microscope
On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 250nm.
a.) What is the limit of resolution on this microscope? Please be specific to how you came up with this answer, what formula did you use?
b.) Will you be able to distinguish 2 points that are 330nm apart as being separate, or will they blur into one?
