You have a cell suspension containing 4 * 10^5 cells/ml and want to seed these cells on microcarriers at a density of 1* 10^4 cells/cm^2. The average diameter of the microcarriers is 157.5um (the diameter range supplied by the vendor is 115-200um).
What is the average surface area of the microcarriers?
What number of microcarriers should you use to obtain an average surface area of 100cm^2?
What volume of your cell suspension should be added to achieve the density given, using the surface area of 100cm^2?