1) The irreversible steps in glycolysis and gluconeogenesis are points for coordinated regulation of flux through these pathways. Which of the isozymes, hexokinase II or hexokinase IV, reaches halfVmax at lower substrate concentrations and how doesthis make sense physiologically?

2) a- What is reciprocal regulation? Describe how this mechanism works to regulate phosphofructokinase-1 (PFK-1) andfructose 1,6-bisphosphatase.

b- Briefly describe the reciprocal trascriptional regulation of insulin on the enzymes of glycolysisand gluconeogenesis.