Studies conducted with a lysozome mutant that contains an Asp->Asn change at position 52 and a Glu->Gln change at position 35 exhibited almost a complete loss in emzymatic activity. What is the most likely explanation for the decrease in emzyme activity in the mutant? a. increased affinity for substrate b. absence of negative charges in the active site c.change in the active site scaffold d. larger amino acids in the active site decrease the affinity for substrate.