Q1. The free energy for the oxidation of glucose to {rm CO_2} and water is -686 kcal/mol and the free energy for the reduction of NAD+ to NADH is +53 kcal/mol. Why are only two molecules of NADH formed all through glycolysis while it appears that as many as a dozen would be formed?

Q2. Sickle cell anemia is a disease of red blood cells in which the genetic mutation in DNA leads to a mutation in hemoglobin. A single base vary alters the DNA sequence CTC to CAC which codes for the wrong amino acid. What amino acid is coded for through the normal DNA sequence, CTC?