1. What is the significance of Rf(relative to front) values? What information can be deduced about the molecules separated by paper chromatography, assuming that you know nothing about each molecule except where it was extractedfrom?

2. Based on your results, what are the optimum wavelengths of light for absorptionby each pigment (hint: look for spikes in the spectrum)? How do theresults compare to the predictions you made? Do your findings correspond with those noted in the literature?

3. If you were to use an aquatic plantfor your starting material, what would you expect to see in terms of pigments and absorption spectra, incomparison to your observed results from spinach? Explain.