(a) When doing RAPD PCR, what are the two important changes from the conditions used for standard PCR?

(b) When doing RT-PCR (starting with RNA template), what are the two important changes from the conditions used for standard PCR?

(c) If you find that you are getting many products when doing a PCR reaction, what are two things you could do to try to achieve cleaner results?

(d) What is isothermal DNA amplification?