1. a. What is meant by the term “clone”?

b. Why is it important to obtain large quantities of a particular DNA sequence?

c. List the steps you would take to obtain a clone of a DNA sequence.

2. For each of the following, give a brief description:

restriction enzyme
vector
recombinant DNA molecule
sticky ends

3. a. What is the process of transformation?

b. What is meant by competent cells?

c. What would you use a selection system for?

4. a. What information could you obtain from a southern blot?

b. What information could you obtain from a northern blot?

c. Describe what a genomic library is.

5. a. You are a forensic scientist and you perform a southern analysis to try and link a possible suspect to the scene of a crime. Draw the results of such a southern analysis and decide if the suspect was present or not.

b. Describe a possible application for making a transgenic animal or plant.