Solution-Explain how will the net charge of a typical
The table below indicates approximate values for these at 25C. For reference, the delta H(ionization) for Tris is 47kJ/mole.
Group deta H (ionization)(kJ/mole)
Asp 3.9
Glu 0
Lys 48
Arg 48
His 28
N-term 40
C- term 0
How will the net charge of a typical protein change upon lowering the temperature from 37C to 4C, based on this information?
What can be done experimentally to compensate for this?