5.00-mL solution aliquots contain PCP were carefully measured into five separate 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of PCP (2.000 µg/mL) were added into each flask: 0.000, 0.500, 1.00, 1.50 and 2.00-mL The fluorimeter readings from each solution are listed below. Calculate the concentration of PCP in the sample.
spike VOl. (mL) |
spike amount (µg) |
spike conc.(µg/mL) |
readout |
0.00 |
0.000 |
0.0000 |
3.26 |
0.500 |
1.000 |
0.02000 |
4.8 |
1.000 |
2.000 |
0.04000 |
6.41 |
1.500 |
3.000 |
0.06000 |
8.02 |
2.000 |
4.000 |
0.08000 |
9.56 |