Relation between melting temperature and g-c content of dna
How various sperm cells would be expected
April 28, 2020Safe and effective for many age types and will benefit
April 28, 2020Relation between melting temperature and g-c content of dna
1) Represent graphically the relation between melting temperature and the G-C content of DNA.
a) Construct relations for dependence of melting temperature of a 30 bp DNA duplex on the G-C content. For series of the DNA chains where we vary the number of G-C pairs, plot how Tm changes as a function of f, the fraction of G-C pairs, ranging from 0.33 (i.e. 10 G-C pairs) to 0.67 (i.e 30 G-C) pairs.
b) From observation, list other factors other than G-C content seem to control Tm?
c) Supposing we have two actin filament 10 microns long linked by 10 myosin motors, how long would be needed to move two filaments past each other 5 microns? How much energy is needed?
