Question about molecular biology

Solution-Which spectral type has the hottest stars
April 16, 2020
Explain how this trait is inherited
April 16, 2020

Question about molecular biology

Assume you infected an E. coli culture with a virulent bacteriophage. Most of the cells lysed, but a few survived – 1×10^-4. You wonder where the resistant bacteria came from. Were they caused by the bacteriophage infection or did they already exist in the bacterial culture? Earlier, for a different experiment, you ha spread a dilute suspension of E. coli onto solid medium in a large Petri dish, and, after seeing that about 10^5 colonies were growing up, you made an imprint of the colonies on that plate and transferred it to three other plates – a process known as replica plating – which creates identical patterns of the colonies on 3 plates. You realize that you can use these plates to distinguish between the two possibilities. You pipette a suspension of the bacteriophage onto each of the 3 replica plates so that the bacteria can be infected. What result do you expect if the bacteriophage cause resistance? What result do you expect if the resistant bacteria pre-exist?