Pcr derived dna fragment, The PCR-derived DNA fragment used
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April 7, 2020Pcr derived dna fragment, The PCR-derived DNA fragment used
The PCR-derived DNA fragment used an insert in pUC19 was amplified by 25 cycles.
The amplification mixture contained, among other components, 1 ng lambda DNA in 50 uL.
If you assume that the PCR amplification process and restriction digestion process are 100% efficient, how many pmol of product (1110 bp fragment) were produced?
How mnay ug of DNA (1110 bp product) were present after amplification.
The 1110 fragment was digested with BamHI and HindIII to form the 784 bp product. How many ug of DNA (the 784 bp product) were formed? Average MW of 1 bp= 660 g/mol
