Question 1

1. Choose the optimal enzyme concentration(s) observed.
1.
50 uls
2.
100 uls
3.
200 uls

Question 2

5. Based on the data at pH 2, what can you surmise happened to the active site?
1.
It stayed the same shape
2.
Its shape changed such that the substrate no longer fit inside
3.
Its shape changed but the substrate still fit inside

Question 3

6. Based pH data, which pH experiment most closely resembles the pH found in most of the human body?
a.
2
b.
7
c.
12

Question 4

7. Choose the optimal temperature for enzyme function observed. Note you will have to create your own graph or log in the milliliters of gas bubbles produced on your own to answer this question.
1.
12 ºC
2.
28 ºC
3.
46 °C

Question 5

All of the following variables were tested on enzyme activity EXCEPT:

pH

temperature

substrate concentration

enzyme concentration

Question 6

8. When a person runs a very high fever they can quickly be in a dangerous situation where the body does not function properly. What is happening to the person’s proteins and enzymes in their body? Note use the proper term (hint see previous questions for the term) to describe what happened to the enzyme.
1.
The high heat of fever causes enzymes to become denatured.
2.
The high heat of fever speeds up enzymes to work more efficiently.
3.
The high heat of fever kills enzymes.
4.
The high heat of fever defragments enzymes.

Question 7

2. In the enzyme concentration experiments why does curve on the graph start to slow down and level off after seven to eight minutes? In other words, what can you guess was happening to the amount of substrate when the reaction started to slow down?
1.
The enzyme stops working after 30 seconds
2.
The substrate is consumed
3.
The enzyme is consumed
4.
The reaction gets too hot

Question 8

3. Choose the optimal pH for enzyme function observed.
a.
2
b.
7
c.
12

Question 9

What is the temperature of the human body in Celcius?

37

46

97.8

100

Question 10

4. What happened to the enzyme at pH 2?
1.
It defragmented
2.
It denatured
3.
It renatured
4.
It didn’t change