Part I
A. Define the following terms:

• monotrichous
• amphitrichous
• lophotrichous
• peritrichous

B. What are the three commonly used techniques to test motility?

C. Why is semi-solid media used to test for motility?
D. Why might it be difficult to observe motility in your wet mount?

E. Why is it important to use a needle rather than an inoculating loop when inoculating a motility tube?
F. Which of your organisms, if any, were you able to observe motility in your wet mount?

G. Which of your organisms, if any, were you able to observe motility in your motility tube?
H. Did your direct and indirect observations of motility show the same results? If they didn’t, why do you think that may have been the case?
Part II

A. What is fermentation?
B. Why it is important not to incubate the fermentation tubes beyond 24 hours?
C. Why phenol red is added to the fermentation tubes?
D. Why do bacteria have differences in the carbohydrates they can ferment?
E. Why does the formation of yellow color indicate fermentation?
F. What information can be gained by running a fermentation series on a
G. particular microbe?
H. What does a dark pink or red color indicate?
I. What is the source of the air bubble that may form in the Durham tube?

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Posted on May 3, 2016Author TutorCategories Question, Questions