Q1. It is experimentally determined that the newly isolated bacterium produces about 2200 proteins when grown on glucose and average molecular weight of these proteins is 41,000 Da. If an average amino acid weighs 110 Da and it takes 3 bp to code for each amino acid, how various base pairs does it take to encode a protein of average molecular weight (such as what is the size of a typical gene in this organism)?

Q2. When Purified Protein Derivative (PPD) isolated from Mycobacterium tuberculosis was injected into the skin of a patient, it caused an area of hardening 1 cm in diameter. How must this PPD test be interpreted? What caused the result?