Find the concentration in mg/ml

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Find the concentration in mg/ml

Question: A 2.5 mg/ml stock solution was diluted as described in the table below and then the absorbance of each dilution was determined and presented in the table below.  Using this information, what is the concentration in mg/ml (to two decimal places) of an unknown sample with an absorbance of 0.41?(Hint:  You will have to calculate the concentration in each diluted tube)

Volume of 2.5 mg/ml Stock                         Volume of Buffer                   Absorbtion

5ml                                                              0ml                                          1.0

4ml                                                              1ml                                            0.8

3ml                                                               2ml                                            0.6

2ml                                                               3ml                                              0.4

1ml                                                               4ml                                           0.2

0ml                                                              5ml                                            0.0

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