Biochemists make stock solutions of reagents and use them to make working concentrations of buffers. Some of these include
A. 1 M Tris-HCl (pH 7.6)
B. 1 M Dithiothreitol?
C. 2 MKCl?
D. 1 M K2HPO4
E. 1 M KH2PO4?
F. 1M Potassium Phosphate (pH 7.2)
G. 10 M NaOH?
H. 1 M ZnSO4?
I. 1 M MgCl2?
J. 0.5 M Na2EDTA.
Describe how to construct these stock solutions. You decide on the final volumes and show your calculations. Note: 1 M ZnSO4, 1 M MgCl2, and 1 M Dithiothreitol should not be greater than 50 ml because they are used sparingly.