Explain heat-fixed-specimen slides of microorganisms

Q1. A hypothetical population of 300 wolves has two alleles, FB and FW, for a locus that codes for fur colour. The table below describes the phenotype of a wolf with each possible genotype, as well the number of individuals in the population with each genotype. Which statements correctly describe the population of wolves?

Q2. What are advantages and disadvantages or observing living-specimens slides and heat-fixed-specimen slides of microorganisms?

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