Determine the value of km
Solution-Explain the difference between portal and normal
April 7, 2020What are the predicted striping pattern phenotypes
April 7, 2020Determine the value of km
The information to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by
CO2 (aq) + H2O (l) –>H+ (aq) + HCO3- (aq)
|
[CO2] (mmol * L^-1) |
R (�µmol * L^-1 * s^-1) |
|
1.25 |
28.74 |
|
2.50 |
48.61 |
|
5.00 |
80.28 |
|
20.00 |
155.59 |
Use these values to determine the value of KM, the Michaelis-Menten constant. Answer the four parts below.
a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the corresponding blank.
[CO2] (mmol * L^-1) graph as R (�µmol * L^-1 * s^-1) graph as
2.50 –> ________ 48.61 –> ________
5.00 –> ________ 80.28 –> ________
b) Calculate the slope using the data points from part (a). Then determine the y-intercept using the second point.
Slope= _______ s y-intercept= ___________ L * s * mmol^-1
c) Use the y-intercept to calculate Rmax.
Rmax=___________ mmol * L^-1 * s^-1
d) Use Rmax and the slope to calculate Km.
Km=___________ mmol * L^-1
