Determine the value of km
The information to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by
CO2 (aq) + H2O (l) –>H+ (aq) + HCO3- (aq)
|
[CO2] (mmol * L^-1) |
R (�µmol * L^-1 * s^-1) |
|
1.25 |
28.74 |
|
2.50 |
48.61 |
|
5.00 |
80.28 |
|
20.00 |
155.59 |
Use these values to determine the value of KM, the Michaelis-Menten constant. Answer the four parts below.
a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the corresponding blank.
[CO2] (mmol * L^-1) graph as R (�µmol * L^-1 * s^-1) graph as
2.50 –> ________ 48.61 –> ________
5.00 –> ________ 80.28 –> ________
b) Calculate the slope using the data points from part (a). Then determine the y-intercept using the second point.
Slope= _______ s y-intercept= ___________ L * s * mmol^-1
c) Use the y-intercept to calculate Rmax.
Rmax=___________ mmol * L^-1 * s^-1
d) Use Rmax and the slope to calculate Km.
Km=___________ mmol * L^-1