Question: A linear fragment of DNA that is 22 Kb is cleaved with the individual restriction enzymes SalI, HindIII and KpnI and then in the pair wise combinations listed below. The sizes of DNA fragments seen on the resulting ethidium bromide stained gel are:
SalI: 11 Kb, 6 Kb, 5 Kb
HindIII: 14 Kb, 8 Kb
KpnI: 16 Kb, 6 Kb
SalI and HindIII: 8 Kb, 6Kb, 5Kb, 3Kb
SalI and KpnI: 11 Kb, 5 Kb, 1 Kb
HindIII and KpnI: 8 Kb, 6 Kb
Determine the restriction map for the linear DNA fragment (ie: figure out where each restriction enzymes’ cut sites are located on the DNA fragment).
Select the correct order in which the restriction enzyme cut sites would occur on the linear DNA fragment beginning at one end of the fragment.
Select one:
a. KpnI, SalI, SalI, HindIII
b. KpnI, SalI, HindIII, SalI
c. HindIII, SalI, KpnI, SalI
d. KpnI, SalI, HindIII