Question1. Why is MgCl2 included in the PCR buffer? MgCl2 is used in the PCR buffer because it help

Question2. If you started a PCR protocol with 10 double stranded molecular of DNA, how many double stranded molecules of product would you have after 5 cycles of PCR?

Question3. Where is the initiation and stop codon of the GFPuv gene in the forward and reverse primers, respectively?

Question4. What kinds of materials obtained from a crime scene might contain DNA, and where might you find them at a crime scene?

Question5. Why do you need to perform PCR on DNA obtained froma crime scene?

Question6. What is genotype?

Question7. What is the different between an allele and a locus?

Question8. What is an allele ladder? What is its function in DNA profiling?

Question9. Why do forensic labs analyze non-coding DNA and not genes?

Question10. How unit is defined for the restriction enzymes EcoRI and HindIII from New England BioLabs?

Question11. What is the star activity of restriction enzymes?