Determine cotransduction frequency of thi and pro

Question: The generalized-transduction phage P1 is grown in a pur- pro+ thi+ donor strain. A recipient strain that is pur+ pro- thi- is infected and the donor allele thi+ is selected after transduction. 100 thi+ transductants are then scored for the other alleles. The results are shown below:

pro+ pur- 94

pro+ pur+ 62

pro- pur+ 34

pro- pur- 10

A) What is the cotransduction frequency of thi and pro?

B) What is the cotransduction frequency of thi and pur?

C) From these cotransduction frequencies, what is the gene order?

 

Solution

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