Q1. The concentration of LIN-14 protein, high during the L1 larval stages and decreasing in later stages, regulates the timing of cell divisions in the T-cell lineage in C. elegans. According to the hypothesis presented, lin-4 RNA regulates the level of LIN-14 protein by binding to the lin-14 mRNA and preventing translation. If you could introduce extra copies of lin-4 into the cells and more lin-4 RNA was produced, what effect do you think the extra copies of lin-4 would have on translation of LIN- 14? What effect might this have on cell lineages during development?”

Q2. Describe the physical changes that would occur in SIM medium if you inoculated an organism that was negative for hydrogen sulfide but positive for indole production and motility.