Define the neurospora dna and plasmid
Solution-Describe the specific linkage arrangement
April 8, 2020Solution-Moist soils with high salt concentrations
April 8, 2020Define the neurospora dna and plasmid
A kan-r, tet-r plasmid is treated with the restriction enzymeBglI, which cleaves the kan (kanamycin) gene. The DNA is annealed and ligated to a BglI digest of Neurospora DNA and thenused to transform E. coli.
a. What anitbiotic would you put in the growth medium to ensure that each colony has the plasmid?
b. What antibiotic-resistance phenotypes will be foundamong the resulting colonies?
c. Which phenotype will contain Neurospora DNA inserts?
