Question: Plasmid size of pGLO is 5371 bp and the restriction sites for PstI are 2106 and 3181. Compute to predict the sizes of fragments when gPLO is digested with PstI. Please show work.

Question: A microbiologist wants to make bacteria produce a foreign protein. He has cloned the protein gene into a plasmid. Which is the next step?

A. The plasmid is inserted into the main bacterial chromosome.
B. The plasmid multiplies and produces the protein outside of the bacterium.
C. The bacterial chromosome should be genetically engineered. The plasmid is just used to help the bacterium replicate.
D. The plasmid is taken up by the bacterium, the plasmid replicates, and the protein is produced inside the bacterium.
E. The bacterial genome and the plasmid are inserted into the genome of the species that contains the desired gene.