1 Chambers A and B are separated by a membrane. Chamber A contains 7.22 M KNO3 and chamber B contains 3.89 M KNO3. If K+ is in equilibrium across the membrane, what is the electrical (potential) difference (Resting membrane potential)? Which side is electrically positive?
I am really confused, I calculated it with the Nernst Equation, but I am sure it is wrong…
Ek=61/1*log10 (3.89/7.22)=-16.38mV
The side of Chambers B is electrically positive?
Cl- )Intracellular concentration :4 Extracellular concentration:25 conductance:0.15
Na+ )Intracellular concentration :100 Extracellular concentration:7 conductance:0.21
K+ )Intracellular concentration :110 Extracellular concentration:110 conductance:0.10
I used GHK equation and got -26.66mV (I don’t know if it is right)
Also how do I know if the inside is negative or positive relative to the outside?