You come across a membrane protein that has been determined to be involved with the passive transport process.
A gene has been identified for the protein however you are unable to develop any structural information about this protein by crystallography, CD or NMR.

1. Of the two types of structures that are associated with membrane proteins, which would you presume is most associated with this process and why?

2. Since a structure cannot be determine by the methods above, use the primary sequence of the protein to describe an approach that can be used to confirm or deny that hypothesis.

3. Describe two challenges that are associated with membrane proteins which make the structural studies difficult.