Unlike microscopy, cell fractionation and protein purification is often performed as a series of linked steps that can often take several days or longer than a week’s time.

a. Most buffer solutions used in this process are isotonic and contain protease inhibitors. Why would this be the case?

b. EGTA and some types of protease are commonly used for cell dissociation. Why are each important and why do some cells dissociate from each other more easily than others?