Which nucleotide has been added and which deleted

1. What sequence of nucleotide pairs in a Drosophila gene will encode the amino acid sequence met-trp-phe-trp-met (reading from the amino terminus to the carboxyl terminus)?

2. A wild type gene contains the trinucleotide-pair sequence:
was 5′-GAG-3′ changed to:5′-GTG-3′
3’CTC -5′ 3′-CAC-5′
If the second base pair in this gene segment will change from A-T to T-A, will it still code the glutamic acid?

3. A single nucleotide addition followed by a single nucleotide deletion approximately 20 bp apart in the DNA causes a change in the protein sequence from

-His-Thr-Glu-Asp-Trp-Leu-His-Gln-Asp-

to -His-Asp-Arg-Gly-Leu-Ala-Thr-Ser-Asp-

Which nucleotide has been added and which deleted? What are the original and the new mRNA sequences?

Solution

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