What proportion of spores to expect to produce a colony

Solution-Problem based on amino acids
April 17, 2020
Explain how an ectopic pregnancy occurs
April 17, 2020

What proportion of spores to expect to produce a colony

Suppose that the following enzymatic pathway is used to synthesize amino acid histidine in Neurospora

enzyme A enzyme B enzyme C

Precursor ————> Imidazole ———> Histidinol ———–> Histidine

These enzymes are encoded by three unlinked genes A, B and C respectively. A prototrophic strain with the genotyp ABC is crossed to an auxotroph (triple mutant abc).

 

A. What proportion of spores do you expect to produce a colony on a minimal medium without any of these three substances?

B. What proportion of spores do you expect to produce a colony on a minimal medium supplemented with histidinol?

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