1. What part of the Boil buffer helps “break open” the bacterial cells?

2. What is the difference between bacterial genomic DNA and plasmid DNA that results in the precipitation of genomic DNA with proteins, leaving the plasmid in the supernatant?

3. Restriction endonucleases are especially useful if they generate “sticky” ends. What makes an end “sticky” and why is this especially useful for inserting DNA fragments into plasmid DNA?

4. What specific property of DNA allows it to be seperated by size using a gel electrophoresis system?