What is the percentage of cytochrome
Solution-Formulation of medium used smaller amount of
April 17, 2020Name the six general types of bones in the human body
April 17, 2020What is the percentage of cytochrome
The purity of cytochrome c can be evaluated by the ratio of the molar extinction coefficient (129.1 mM-1cm-1) at 416 nm of the reduced cytochrome c and the molar extinction coefficient (23.2 mM-1cm-1) at 280 of the oxidized cytochrome c. You extracted cytochrome c from yeast in the laboratory and after running UV spectra for the reduced and oxidized cytochrome c you had absorbance of 1.28 and 0.307 units respectively.
(i) What is the percentage of cytochrome c from your experiment?
(ii) If you used a 1 cm cuvette, what is the concentration of your cytochrome c?
