What is the association constant for the peptide
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April 11, 2020What is the association constant for the peptide
The following kon and koff rates for the interaction of antibody (IgG) and a 40 amino acid peptide were determined via surface plasmon resonance (a Biacore machine) to be: kon = 210 nM-1·sec-1, koff=11,000 sec-1
(a) What is the dissociation constant for the peptide:IgG interaction?
(b) What is the association constant for the peptide:IgG interaction?
(c) If the binding study were performed at 298K, what is the ?G (in kJ/mol) of binding?
